PlantImRecepV1, Main, Exploration, bibRecord, 000067

Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.

Identifieur interne : 000067 ( Main/Exploration ); précédent : 000066; suivant : 000068

Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.

Auteurs : Donghyuk Lee [États-Unis] ; Neeraj K. Lal [États-Unis] ; Zuh-Jyh Daniel Lin [États-Unis] ; Shisong Ma [États-Unis, République populaire de Chine] ; Jun Liu [États-Unis, République populaire de Chine] ; Bardo Castro [États-Unis] ; Tania Toru O [États-Unis] ; Savithramma P. Dinesh-Kumar [États-Unis] ; Gitta Coaker [États-Unis]

Source :

Nature communications [ 2041-1723 ] ; 2020.

RBID : pubmed:32296066

Descripteurs français

English descriptors

KwdEn :
- Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Gene Expression Regulation, Plant (genetics), Gene Expression Regulation, Plant (physiology), NADPH Oxidases (genetics), NADPH Oxidases (metabolism), Phosphorylation (genetics), Phosphorylation (physiology), Plant Diseases (genetics), Plant Immunity (genetics), Plant Immunity (physiology), Protein Domains (genetics), Protein Domains (physiology), Protein-Serine-Threonine Kinases (genetics), Protein-Serine-Threonine Kinases (metabolism), Reactive Oxygen Species (metabolism), Signal Transduction (genetics), Signal Transduction (physiology), Ubiquitination (genetics), Ubiquitination (physiology).
MESH :
- chemical , genetics : Arabidopsis Proteins, NADPH Oxidases, Protein-Serine-Threonine Kinases.
- chemical , metabolism : Arabidopsis Proteins, NADPH Oxidases, Protein-Serine-Threonine Kinases, Reactive Oxygen Species.
- genetics : Gene Expression Regulation, Plant, Phosphorylation, Plant Diseases, Plant Immunity, Protein Domains, Signal Transduction, Ubiquitination.
- physiology : Gene Expression Regulation, Plant, Phosphorylation, Plant Immunity, Protein Domains, Signal Transduction, Ubiquitination.

Abstract

Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive. Here we show that RBOHD is regulated by C-terminal phosphorylation and ubiquitination. Genetic and biochemical analyses reveal that the PBL13 receptor-like cytoplasmic kinase phosphorylates RBOHD's C-terminus and two phosphorylated residues (S862 and T912) affect RBOHD activity and stability, respectively. Using protein array technology, we identified an E3 ubiquitin ligase PIRE (PBL13 interacting RING domain E3 ligase) that interacts with both PBL13 and RBOHD. Mimicking phosphorylation of RBOHD (T912D) results in enhanced ubiquitination and decreased protein abundance. PIRE and PBL13 mutants display higher RBOHD protein accumulation, increased ROS production, and are more resistant to bacterial infection. Thus, our study reveals an intricate post-translational network that negatively regulates the abundance of a conserved NADPH oxidase.

DOI: 10.1038/s41467-020-15601-5
PubMed: 32296066
PubMed Central: PMC7160206

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000161
to stream Main, to step Curation: 000161

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.</title>
<author><name sortKey="Lee, Donghyuk" sort="Lee, Donghyuk" uniqKey="Lee D" first="Donghyuk" last="Lee">Donghyuk Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lal, Neeraj K" sort="Lal, Neeraj K" uniqKey="Lal N" first="Neeraj K" last="Lal">Neeraj K. Lal</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Zuh Jyh Daniel" sort="Lin, Zuh Jyh Daniel" uniqKey="Lin Z" first="Zuh-Jyh Daniel" last="Lin">Zuh-Jyh Daniel Lin</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Donald Danforth Plant Science Center, St Louis, MO, 63132, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Donald Danforth Plant Science Center, St Louis, MO, 63132</wicri:regionArea>
<wicri:noRegion>63132</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ma, Shisong" sort="Ma, Shisong" uniqKey="Ma S" first="Shisong" last="Ma">Shisong Ma</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Life Sciences, University of Science and Technology of China, 230027, Hefei</wicri:regionArea>
<placeName><settlement type="city">Hefei</settlement>
<region type="province">Anhui</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Liu, Jun" sort="Liu, Jun" uniqKey="Liu J" first="Jun" last="Liu">Jun Liu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Castro, Bardo" sort="Castro, Bardo" uniqKey="Castro B" first="Bardo" last="Castro">Bardo Castro</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Toru O, Tania" sort="Toru O, Tania" uniqKey="Toru O T" first="Tania" last="Toru O">Tania Toru O</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dinesh Kumar, Savithramma P" sort="Dinesh Kumar, Savithramma P" uniqKey="Dinesh Kumar S" first="Savithramma P" last="Dinesh-Kumar">Savithramma P. Dinesh-Kumar</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Coaker, Gitta" sort="Coaker, Gitta" uniqKey="Coaker G" first="Gitta" last="Coaker">Gitta Coaker</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA. glcoaker@ucdavis.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2020">2020</date>
<idno type="RBID">pubmed:32296066</idno>
<idno type="pmid">32296066</idno>
<idno type="doi">10.1038/s41467-020-15601-5</idno>
<idno type="pmc">PMC7160206</idno>
<idno type="wicri:Area/Main/Corpus">000161</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000161</idno>
<idno type="wicri:Area/Main/Curation">000161</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000161</idno>
<idno type="wicri:Area/Main/Exploration">000161</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.</title>
<author><name sortKey="Lee, Donghyuk" sort="Lee, Donghyuk" uniqKey="Lee D" first="Donghyuk" last="Lee">Donghyuk Lee</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lal, Neeraj K" sort="Lal, Neeraj K" uniqKey="Lal N" first="Neeraj K" last="Lal">Neeraj K. Lal</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Zuh Jyh Daniel" sort="Lin, Zuh Jyh Daniel" uniqKey="Lin Z" first="Zuh-Jyh Daniel" last="Lin">Zuh-Jyh Daniel Lin</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Donald Danforth Plant Science Center, St Louis, MO, 63132, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Donald Danforth Plant Science Center, St Louis, MO, 63132</wicri:regionArea>
<wicri:noRegion>63132</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ma, Shisong" sort="Ma, Shisong" uniqKey="Ma S" first="Shisong" last="Ma">Shisong Ma</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>School of Life Sciences, University of Science and Technology of China, 230027, Hefei</wicri:regionArea>
<placeName><settlement type="city">Hefei</settlement>
<region type="province">Anhui</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Liu, Jun" sort="Liu, Jun" uniqKey="Liu J" first="Jun" last="Liu">Jun Liu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Castro, Bardo" sort="Castro, Bardo" uniqKey="Castro B" first="Bardo" last="Castro">Bardo Castro</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Toru O, Tania" sort="Toru O, Tania" uniqKey="Toru O T" first="Tania" last="Toru O">Tania Toru O</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dinesh Kumar, Savithramma P" sort="Dinesh Kumar, Savithramma P" uniqKey="Dinesh Kumar S" first="Savithramma P" last="Dinesh-Kumar">Savithramma P. Dinesh-Kumar</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Coaker, Gitta" sort="Coaker, Gitta" uniqKey="Coaker G" first="Gitta" last="Coaker">Gitta Coaker</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA. glcoaker@ucdavis.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616</wicri:regionArea>
<wicri:noRegion>95616</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Nature communications</title>
<idno type="eISSN">2041-1723</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis Proteins (genetics)</term>
<term>Arabidopsis Proteins (metabolism)</term>
<term>Gene Expression Regulation, Plant (genetics)</term>
<term>Gene Expression Regulation, Plant (physiology)</term>
<term>NADPH Oxidases (genetics)</term>
<term>NADPH Oxidases (metabolism)</term>
<term>Phosphorylation (genetics)</term>
<term>Phosphorylation (physiology)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Immunity (genetics)</term>
<term>Plant Immunity (physiology)</term>
<term>Protein Domains (genetics)</term>
<term>Protein Domains (physiology)</term>
<term>Protein-Serine-Threonine Kinases (genetics)</term>
<term>Protein-Serine-Threonine Kinases (metabolism)</term>
<term>Reactive Oxygen Species (metabolism)</term>
<term>Signal Transduction (genetics)</term>
<term>Signal Transduction (physiology)</term>
<term>Ubiquitination (genetics)</term>
<term>Ubiquitination (physiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Domaines protéiques (génétique)</term>
<term>Domaines protéiques (physiologie)</term>
<term>Espèces réactives de l'oxygène (métabolisme)</term>
<term>Immunité des plantes (génétique)</term>
<term>Immunité des plantes (physiologie)</term>
<term>Maladies des plantes (génétique)</term>
<term>NADPH oxidase (génétique)</term>
<term>NADPH oxidase (métabolisme)</term>
<term>Phosphorylation (génétique)</term>
<term>Phosphorylation (physiologie)</term>
<term>Protein-Serine-Threonine Kinases (génétique)</term>
<term>Protein-Serine-Threonine Kinases (métabolisme)</term>
<term>Protéines d'Arabidopsis (génétique)</term>
<term>Protéines d'Arabidopsis (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (physiologie)</term>
<term>Transduction du signal (génétique)</term>
<term>Transduction du signal (physiologie)</term>
<term>Ubiquitination (génétique)</term>
<term>Ubiquitination (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term>
<term>NADPH Oxidases</term>
<term>Protein-Serine-Threonine Kinases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arabidopsis Proteins</term>
<term>NADPH Oxidases</term>
<term>Protein-Serine-Threonine Kinases</term>
<term>Reactive Oxygen Species</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Phosphorylation</term>
<term>Plant Diseases</term>
<term>Plant Immunity</term>
<term>Protein Domains</term>
<term>Signal Transduction</term>
<term>Ubiquitination</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Domaines protéiques</term>
<term>Immunité des plantes</term>
<term>Maladies des plantes</term>
<term>NADPH oxidase</term>
<term>Phosphorylation</term>
<term>Protein-Serine-Threonine Kinases</term>
<term>Protéines d'Arabidopsis</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Transduction du signal</term>
<term>Ubiquitination</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Espèces réactives de l'oxygène</term>
<term>NADPH oxidase</term>
<term>Protein-Serine-Threonine Kinases</term>
<term>Protéines d'Arabidopsis</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Domaines protéiques</term>
<term>Immunité des plantes</term>
<term>Phosphorylation</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Transduction du signal</term>
<term>Ubiquitination</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Phosphorylation</term>
<term>Plant Immunity</term>
<term>Protein Domains</term>
<term>Signal Transduction</term>
<term>Ubiquitination</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive. Here we show that RBOHD is regulated by C-terminal phosphorylation and ubiquitination. Genetic and biochemical analyses reveal that the PBL13 receptor-like cytoplasmic kinase phosphorylates RBOHD's C-terminus and two phosphorylated residues (S862 and T912) affect RBOHD activity and stability, respectively. Using protein array technology, we identified an E3 ubiquitin ligase PIRE (PBL13 interacting RING domain E3 ligase) that interacts with both PBL13 and RBOHD. Mimicking phosphorylation of RBOHD (T912D) results in enhanced ubiquitination and decreased protein abundance. PIRE and PBL13 mutants display higher RBOHD protein accumulation, increased ROS production, and are more resistant to bacterial infection. Thus, our study reveals an intricate post-translational network that negatively regulates the abundance of a conserved NADPH oxidase.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32296066</PMID>
<DateCompleted><Year>2020</Year>
<Month>07</Month>
<Day>24</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>08</Month>
<Day>06</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2041-1723</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>11</Volume>
<Issue>1</Issue>
<PubDate><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>Nature communications</Title>
<ISOAbbreviation>Nat Commun</ISOAbbreviation>
</Journal>
<ArticleTitle>Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.</ArticleTitle>
<Pagination><MedlinePgn>1838</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/s41467-020-15601-5</ELocationID>
<Abstract><AbstractText>Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS production during innate immunity. However, how RBOHD is negatively regulated remains elusive. Here we show that RBOHD is regulated by C-terminal phosphorylation and ubiquitination. Genetic and biochemical analyses reveal that the PBL13 receptor-like cytoplasmic kinase phosphorylates RBOHD's C-terminus and two phosphorylated residues (S862 and T912) affect RBOHD activity and stability, respectively. Using protein array technology, we identified an E3 ubiquitin ligase PIRE (PBL13 interacting RING domain E3 ligase) that interacts with both PBL13 and RBOHD. Mimicking phosphorylation of RBOHD (T912D) results in enhanced ubiquitination and decreased protein abundance. PIRE and PBL13 mutants display higher RBOHD protein accumulation, increased ROS production, and are more resistant to bacterial infection. Thus, our study reveals an intricate post-translational network that negatively regulates the abundance of a conserved NADPH oxidase.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lee</LastName>
<ForeName>DongHyuk</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lal</LastName>
<ForeName>Neeraj K</ForeName>
<Initials>NK</Initials>
<AffiliationInfo><Affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Zuh-Jyh Daniel</ForeName>
<Initials>ZD</Initials>
<AffiliationInfo><Affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Donald Danforth Plant Science Center, St Louis, MO, 63132, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ma</LastName>
<ForeName>Shisong</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>School of Life Sciences, University of Science and Technology of China, 230027, Hefei, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Jun</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Castro</LastName>
<ForeName>Bardo</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Toruño</LastName>
<ForeName>Tania</ForeName>
<Initials>T</Initials>
<Identifier Source="ORCID">0000-0002-9220-016X</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dinesh-Kumar</LastName>
<ForeName>Savithramma P</ForeName>
<Initials>SP</Initials>
<AffiliationInfo><Affiliation>Department of Plant Biology and the Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Coaker</LastName>
<ForeName>Gitta</ForeName>
<Initials>G</Initials>
<Identifier Source="ORCID">0000-0003-0899-2449</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA, 95616, USA. glcoaker@ucdavis.edu.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>R01 GM092772</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R25 GM056765</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>R35 GM136402</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
<Grant><GrantID>S10 OD021801</GrantID>
<Acronym>OD</Acronym>
<Agency>NIH HHS</Agency>
<Country>United States</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>04</Month>
<Day>15</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Nat Commun</MedlineTA>
<NlmUniqueID>101528555</NlmUniqueID>
<ISSNLinking>2041-1723</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.6.-</RegistryNumber>
<NameOfSubstance UI="C568857">respiratory burst oxidase homolog D, Arabidopsis</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.6.3.-</RegistryNumber>
<NameOfSubstance UI="D019255">NADPH Oxidases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber>
<NameOfSubstance UI="C000610845">PBL13 protein, Arabidopsis</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber>
<NameOfSubstance UI="D017346">Protein-Serine-Threonine Kinases</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019255" MajorTopicYN="N">NADPH Oxidases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010766" MajorTopicYN="N">Phosphorylation</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057865" MajorTopicYN="N">Plant Immunity</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000072417" MajorTopicYN="N">Protein Domains</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017346" MajorTopicYN="N">Protein-Serine-Threonine Kinases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054875" MajorTopicYN="N">Ubiquitination</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>04</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
<Month>03</Month>
<Day>09</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>4</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>4</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>7</Month>
<Day>25</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">32296066</ArticleId>
<ArticleId IdType="doi">10.1038/s41467-020-15601-5</ArticleId>
<ArticleId IdType="pii">10.1038/s41467-020-15601-5</ArticleId>
<ArticleId IdType="pmc">PMC7160206</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>BMC Plant Biol. 2014 Dec 19;14:374</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25522736</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2015 Aug;56(8):1472-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25941234</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2014 Mar 12;15(3):329-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24629339</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2006 Mar 09;7:123</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16526949</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2010 Mar 18;464(7287):418-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20164835</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2018 Sep 12;24(3):379-391.e5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30212650</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Immunol. 2016 Sep;16(9):537-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27477127</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2016 Apr;210(2):627-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26765243</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):517-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11756663</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2007 Mar;19(3):1065-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17400895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2018 May 31;173(6):1454-1467.e15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29656896</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 2012 Jan;17(1):9-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22037416</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Integr Plant Biol. 2013 Jan;55(1):54-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23137267</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2009 Jun 18;459(7249):996-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19494811</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2012 Aug;159(4):1845-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22730426</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Physiol Rev. 2017 Jan;97(1):253-281</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27932395</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Protoc Plant Biol. 2016 May;1(1):217-234</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31725992</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2015 Mar;27(3):839-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25770109</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2011 Mar 8;21(5):345-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21333538</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2017 Feb;29(2):243-259</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28100709</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2011 Jul;156(3):1364-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21546454</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2012 Apr;11(4):M111.014555</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22215637</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2014 May 19;24(10):R453-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24845678</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2017 Dec 22;8(1):2265</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29273780</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2011 Jun 17;332(6036):1439-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21680842</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 May 21;110(21):8744-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23650383</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2018 Apr 11;23(4):485-497.e5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29649442</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2014 Apr 10;54(1):43-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24630626</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2007;2(7):1565-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17585298</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2018 Jul;30(7):1543-1561</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29871986</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2017 Aug;38:124-132</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28538164</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2009 Jun 30;7(6):e1000139</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19564897</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2020 Apr;32(4):1063-1080</Citation>
<ArticleIdList><ArticleId IdType="pubmed">32034035</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Signal. 2009 Aug 18;2(84):ra45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19690331</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genetics. 1995 Dec;141(4):1597-604</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8601497</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Biosci (Elite Ed). 2009 Jun 01;1:142-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19482632</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2008 Apr 4;283(14):8885-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18218618</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2011 Dec;14(6):691-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21862390</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Integr Plant Biol. 2018 Sep;60(9):827-840</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29877613</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2006 Feb 24;124(4):803-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16497589</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2013 Jul;10(7):676-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23749301</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2013 Nov 1;342(6158):624-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24114786</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2005 Aug;8(4):397-403</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15939662</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2006;1(6):2856-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17406544</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2015 Jul;27(7):2042-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26198070</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2010 Mar;10(6):1265-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20077414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2014 Jan 20;24(2):134-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24388849</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2004 Nov-Dec;3(6):1234-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15595733</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2006 Nov 16;444(7117):323-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17108957</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2017 Jun;214(4):1646-1656</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28195333</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2016 Aug 05;12(8):e1005811</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27494702</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2016 Nov 15;35(22):2468-2483</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27679653</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2007 Dec 14;28(5):730-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18082598</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2014 Apr 22;26(4):1729-1745</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24755455</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2011 Sep;23(9):3374-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21949153</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2018 Feb 20;115(8):E1906-E1915</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29432171</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2017 Jul;215(1):382-396</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28513921</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Phytopathol. 2017 Aug 4;55:109-137</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28525309</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Immunol. 2005 Oct;6(10):973-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16177805</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2007 Jul 26;448(7152):497-500</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17625569</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2009 May 29;137(5):860-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19490895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2015 Dec;169(4):2950-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26432875</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Biol. 1997 Jan;200(Pt 1):1-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9023991</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2016 Mar;67(6):1663-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26798024</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):11034-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27651493</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Anhui</li>
</region>
<settlement><li>Hefei</li>
<li>Pékin</li>
</settlement>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Lee, Donghyuk" sort="Lee, Donghyuk" uniqKey="Lee D" first="Donghyuk" last="Lee">Donghyuk Lee</name>
</noRegion>
<name sortKey="Castro, Bardo" sort="Castro, Bardo" uniqKey="Castro B" first="Bardo" last="Castro">Bardo Castro</name>
<name sortKey="Coaker, Gitta" sort="Coaker, Gitta" uniqKey="Coaker G" first="Gitta" last="Coaker">Gitta Coaker</name>
<name sortKey="Dinesh Kumar, Savithramma P" sort="Dinesh Kumar, Savithramma P" uniqKey="Dinesh Kumar S" first="Savithramma P" last="Dinesh-Kumar">Savithramma P. Dinesh-Kumar</name>
<name sortKey="Lal, Neeraj K" sort="Lal, Neeraj K" uniqKey="Lal N" first="Neeraj K" last="Lal">Neeraj K. Lal</name>
<name sortKey="Lin, Zuh Jyh Daniel" sort="Lin, Zuh Jyh Daniel" uniqKey="Lin Z" first="Zuh-Jyh Daniel" last="Lin">Zuh-Jyh Daniel Lin</name>
<name sortKey="Lin, Zuh Jyh Daniel" sort="Lin, Zuh Jyh Daniel" uniqKey="Lin Z" first="Zuh-Jyh Daniel" last="Lin">Zuh-Jyh Daniel Lin</name>
<name sortKey="Liu, Jun" sort="Liu, Jun" uniqKey="Liu J" first="Jun" last="Liu">Jun Liu</name>
<name sortKey="Ma, Shisong" sort="Ma, Shisong" uniqKey="Ma S" first="Shisong" last="Ma">Shisong Ma</name>
<name sortKey="Toru O, Tania" sort="Toru O, Tania" uniqKey="Toru O T" first="Tania" last="Toru O">Tania Toru O</name>
</country>
<country name="République populaire de Chine"><region name="Anhui"><name sortKey="Ma, Shisong" sort="Ma, Shisong" uniqKey="Ma S" first="Shisong" last="Ma">Shisong Ma</name>
</region>
<name sortKey="Liu, Jun" sort="Liu, Jun" uniqKey="Liu J" first="Jun" last="Liu">Jun Liu</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PlantImRecepV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000067 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000067 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PlantImRecepV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:32296066
   |texte=   Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:32296066" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PlantImRecepV1

This area was generated with Dilib version V0.6.38.
Data generation: Sat Nov 21 12:33:18 2020. Site generation: Sat Nov 21 12:33:47 2020

	Serveur d'exploration sur les récepteurs immunitaires végétaux
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les récepteurs immunitaires végétaux

Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.

Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki